Journal: Annals of Translational Medicine

Article Title: Optimization of a microfluidics-based next generation sequencing assay for clinical oncology diagnostics

doi: 10.21037/atm.2018.05.07

Figure Lengend Snippet: Characterization of sequencing depth by sample type, input amount, and across individual PCR amplicons. (A) Box plots demonstrate the distribution of maximum (red) and minimum (blue) read depth for both formalin fixed (upper) and unfixed (lower) specimens at indicated DNA mass inputs using the integrated fluidics circuit (IFC) library preparation; (B) mean coverage by amplicon for fixed samples with 15 ng of DNA input on the IFC.

Article Snippet: Further, we noted a broad distribution of mean coverage depth by amplicon from approximately 500× to 18,000× on the IFC assay ( ); over half of the amplicons within the assay demonstrated a mean coverage of >8,000×. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 3 caption a7 Characterization of sequencing depth by sample type, input amount, and across individual PCR amplicons. (A) Box plots demonstrate the distribution of maximum (red) and minimum (blue) read depth for both formalin fixed (upper) and unfixed (lower) specimens at indicated DNA mass inputs using the integrated fluidics circuit (IFC) library preparation; (B) mean coverage by amplicon for fixed samples with 15 ng of DNA input on the IFC.

Techniques: Sequencing, Amplification

Journal: Annals of Translational Medicine

Article Title: Optimization of a microfluidics-based next generation sequencing assay for clinical oncology diagnostics

doi: 10.21037/atm.2018.05.07

Figure Lengend Snippet: Distribution of the total number of variants called per sample with IFC library preparation at various DNA inputs. Box plots show the distribution in the total number of single nucleotide variants (upper) and indel (lower) variants called for both fixed (red) and unfixed (blue) specimens at the indicated DNA inputs. A total of 115 replicates from 30 biologically unique samples are represented. IFC, integrated fluidics circuit.

Article Snippet: Further, we noted a broad distribution of mean coverage depth by amplicon from approximately 500× to 18,000× on the IFC assay ( ); over half of the amplicons within the assay demonstrated a mean coverage of >8,000×. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 3 caption a7 Characterization of sequencing depth by sample type, input amount, and across individual PCR amplicons. (A) Box plots demonstrate the distribution of maximum (red) and minimum (blue) read depth for both formalin fixed (upper) and unfixed (lower) specimens at indicated DNA mass inputs using the integrated fluidics circuit (IFC) library preparation; (B) mean coverage by amplicon for fixed samples with 15 ng of DNA input on the IFC.

Techniques: